专业从事离子液体的合成、应用及其相关技术的研究工作----Lanzhou Greenchem ILs/青岛奥立科

题名

[Wiley数据库][2008] Sensitive, label-free protein assay using 1-ethyl-3-methylimidazolium tetrafluoroborate-supported microchip electrophoresis with laser-induced fluorescence detection

参考引用方式

Yuanhong Xu, Jing Li, Erkang Wang. Electrophoresis 2008, 29, 1852–1858

研究机构

中科院长春应用化学研究所

所涉及离子液体

1-乙基-3-甲基咪唑四氟硼酸盐  143314-16-3

研究领域

微芯片电泳；无标记蛋白质分析；激光诱导荧光检测

论文摘要

Based on the dimer–monomer equilibrium movement of the fluorescent dye Pyronin Y(PY), a rapid, simple, highly sensitive, label-free method for protein detection was developed by microchip electrophoresis with LIF detection. PY formed a nonfluorescent dimer induced by the premicellar aggregation of an anionic surfactant, SDS, however, the fluorescence intensity of the system increased dramatically when proteins such as BSA, bovine hemoglobin, cytochrome c, and trypsin were added to the solution due to the transition of dimer to fluorescent monomer. Furthermore, 1-ethyl-3-methylimidazolium tetrafluoroborate (EMImBF4) instead of PBS was applied as running buffers in microchip electrophoresis. Due to the excellent properties of EMImBF4, not only nonspecific protein adsorption was more efficiently suppressed, but also approximately ten-fold higher fluorescence intensity enhancement was obtained than that using PBS. Under the optimal conditions, detection limits for BSA, bovine hemoglobin, cytochrome c, and trypsin were 1.0*10^-6, 2*10-6, 7*10^-7, and 5*10^-7 mg/mL, respectively. Thus, without covalent modification of the protein, a protein assay method with high sensitivity was achieved on microchips.

关键词

ionic liquid / Label-free protein assay / Microchip / Protein