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[Wiley数据库][2012]Electrochemical DNA Biosensor Based on Graphene and TiO2 Nanorods Composite Film for
发布时间:2016-03-15 22:08:26 阅读次数:
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题名
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[Wiley数据库][2012]Electrochemical DNA Biosensor Based on Graphene and TiO2 Nanorods Composite Film for the Detection of Transgenic Soybean Gene Sequence of MON89788
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参考引用方式
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Hongwei Gao, Min Sun, Chao Lin, Shubai Wang. Electroanalysis 2012, 24, No. 12, 2283–2290
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研究机构
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山东出入境检验检疫局；青岛农业大学
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所涉及离子液体
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N-丁基吡啶六氟磷酸盐 186088-50-6
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研究领域
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电化学DNA生物传感器；基因序列检测；石墨烯TiO2纳米复合膜
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论文摘要
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Based on graphene (GR), TiO2 nanorods, and chitosan (CTS) nanocomposite modified carbon ionic liquid electrode (CILE) as substrate electrode, a new electrochemical DNA biosensor was effectively fabricated for the detection of the transgenic soybean sequence of MON89788. By using methylene blue (MB) as hybridization indicator for monitoring the hybridization with different ssDNA sequences, the differential pulse voltammetric response of MB on DNA modified electrodes were recorded and compared. Due to the synergistic effects of TiO2 nanorods and GR on the electrode surface, the electrochemical responses of MB were greatly increased. Under optimal conditions the differential pulse voltammetric response of the target ssDNA sequence could be detected in the range from 1.0×10-12 to 1.0×10-6 mol/L with a detection limit of 7.21×10-13 mol/L (3σ). This electrochemical DNA biosensor was further applied to the polymerase chain reaction (PCR) product of transgenic soybeans with satisfactory results.
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关键词
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TiO2 nanorods, Graphene, Chitosan, Carbon ionic liquid electrode, Electrochemical DNA biosensor
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