有机合成(108) 离子液体(441) 催化(166) 离子液体制备(12) 离子液体物性数据(10) 纳米(30) 分离过程(14) 综述(5) 电化学(57) 离子液体纯化(1) 气体处理(17) 荧光(4) 脱硫(1) 催化制氢(1) 太阳能电池(1) 离子液体性能研究(20) 变焦透镜(6) 纤维素溶解(6) 离子液体理论研究(4) 润滑(10) 添加剂(4) 溶剂(38) 气体压缩(1) 功能材料(42) 脱酸工艺(1) 产业化(30) 物化性质研究(2) 新能源(9) 新型离子液体(11) 会议(18) 分析检测(3) 电池(29) 空间技术(9) 高极性色谱柱(1) 离子液体市场分析(1) 电子技术(6) 生物相容性(9) 分析测试(16) 缩微实验室(1)
[Wiley数据库][2013] 11α hydroxylation of 16α, 17-epoxyprogesterone in biphasic ionic liquid/water syste
发布时间:2016-05-10 20:22:17 阅读次数:
|
题名
|
[Wiley数据库][2013] 11α hydroxylation of 16α, 17-epoxyprogesterone in biphasic ionic liquid/water system by Aspergillus ochraceus
|
|
参考引用方式
|
Shuhong Mao, Boyuan Hua, Na Wang, Xiaojie HU, Zhijiang Ge, Yanqing Li, Sha Liua and Fuping Lu. J Chem Technol Biotechnol 2013; 88: 287–292
|
|
研究机构
|
天津科技大学
|
|
所涉及离子液体
|
|
|
研究领域
|
11α羟基化；16α, 17-环氧黄体酮；IL/水双相体系
|
|
论文摘要
|
The improved efficiency of steroid biotransformation using the biphasic system is generally attributed to the positive effect on the solubility of substrate in aqueous media. A promising alternative for the application of organic solvents in biphasic systems is the use of ionic liquids (ILs). This study aims to investigate the applicability of the biphasic ILs/water syste for 11α hydroxylation of 16α, 17-epoxyprogesterone (HEP) by Aspergillus ochraceus. RESULTS: Of the seven ILs tested, [C3mim][PF6] exhibited the best biocompatibility, with markedly improved biotransformation efficiency. In the [C3mim][PF6]-based biphasic system, substrate conversion reached 90% under the condition in which buffer pH, volume ratio of buffer to ILs, cell concentration, and substrate concentration were 4.8, 10/1, 165 g L−1 and 20 g L−1, respectively. This is more efficient than that of the monophasic aqueous system. The effects of the cations and anions of these ILs on the 11α hydroxylation of 16α, 17-epoxyprogesterone (HEP) by A. ochraceus is also discussed. CONCLUSION: The above results showed that IL/water biphasic system improved the efficiency of 11α hydroxylation of 16α, 17-epoxyprogesterone (HEP) by A.ochraceus, thus suggesting the potential industrial application of ILs-based biphasic systems for steroid biotransformation.
|
|
关键词
|
11α hydroxylation; Aspergillus ochraceus; 16α, 17-epoxyprogesterone; byphasic system; ionic liquids
|
